Cholesterol crystallization in model biles: effects of bile salt and phospholipid species composition
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چکیده
منابع مشابه
Cholesterol crystallization in model biles: effects of bile salt and phospholipid species composition.
Cholesterol in human bile is solubilized in micelles by (relatively hydrophobic) bile salts and phosphatidylcholine (unsaturated acyl chains at sn-2 position). Hydrophilic tauroursodeoxycholate, dipalmitoyl phosphatidylcholine, and sphingomyelin all decrease cholesterol crystal-containing zones in the equilibrium ternary phase diagram (van Erpecum, K. J., and M. C. Carey. 1997. Biochim. Biophys...
متن کاملPhospholipid molecular species influence crystal habits and transition sequences of metastable intermediates during cholesterol crystallization from bile salt-rich model bile.
Despite its importance in cholesterol gallstone formation, crystallization of cholesterol from bile is poorly understood, especially with respect to the influences of other biliary lipids. We reported recently (Konikoff et al. J. Clin. Invest. 1992. 90: 1155-1160) that cholesterol can crystallize from model and native biles as filamentous crystals covered by a surface layer of lecithin molecule...
متن کاملQuantitation of cholesterol-carrying particles in human gallbladder bile.
The inter-mixed micellar/vesicular (non-phospholipid-associated) bile salt concentration (IMC) can be rapidly measured in model biles by centrifugal ultrafiltration, thus allowing reliable separation of vesicular and micellar cholesterol carriers by gel filtration with an elution buffer containing bile salts at the correct IMC (Donovan, J. M., and A. A. Jackson. 1993. J. Lipid Res. 34: 1121-112...
متن کاملImaging and Monitoring Cholesterol Crystallization in Bile
Characterizing the microstructural evolution of lipid aggregates and precipitates in lithogenic bile is pivotal for understanding the process of cholesterol crystallization, leading to cholesterol gallstone formation. We have studied cholesterol precipitation in bile models and in human bile samples by the combined use of lightand cryotransmission electron microscopy with density gradient separ...
متن کاملPartial replacement of bile salts causes marked changes of cholesterol crystallization in supersaturated model bile systems.
Cholesterol crystallization is a key step in gallstone formation and is influenced by numerous factors. Human bile contains various bile salts having different hydrophobicity and micelle-forming capacities, but the importance of lipid composition to bile metastability remains unclear. This study investigated the effect of bile salts on cholesterol crystallization in model bile (MB) systems. Sup...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2001
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)31578-9